Generator

Part:BBa_K143079:Design

Designed by: Chris Hirst   Group: iGEM08_Imperial_College   (2008-10-27)

Pveg-spoVG GFP expression construct



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2225


Design Notes

The 5' and 3' amyE integration sequences were PCR cloned from the B. subtilis integration vector pDR111 using Pfu DNA polymerase. The double terminator, GFP gene and chlorapmphenicol acetyltransferase gene were taken from the registry. The sequence of promoter Pveg and RBS spoVG were taken from papers.


Source

The 5' and 3' amyE integration sequences were PCR cloned from the B. subtilis integration vector pDR111 utilising Pfu DNA polymerase and cloned into a BioBrick with the parts from the registry and the Pveg-spoVG part that was synthesised by GeneArt.